European Journal of Cancer
Volume 39, Issue 13 , Pages 1936-1947, September 2003

Gene expression profiles in human non-small and small-cell lung cancers

  • S. Difilippantonio

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • Both authors contributed equally to this study.
    • Current address: Experimental Immunology Branch, National Cancer Institute/National Institutes of Health, Bethesda, MD, USA.
    • ,
  • Y. Chen

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • Both authors contributed equally to this study.
    • ,
  • A. Pietas

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • ,
  • K. Schlüns

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • ,
  • M. Pacyna-Gengelbach

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • ,
  • N. Deutschmann

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • ,
  • H.M. Padilla-Nash

      Affiliations

    • Genetics Department, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    • ,
  • T. Ried

      Affiliations

    • Genetics Department, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA
    • ,
  • I. Petersen

      Affiliations

    • Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany
    • Corresponding Author InformationCorresponding author. Tel.: +49-30-450-536050; fax: +49-30-450-536110

Received 17 October 2002; received in revised form 6 February 2003; accepted 13 February 2003.

Abstract 

Suppression subtractive hybridisation (SSH) was performed comparing normal bronchial epithelial cells with a lung squamous cell carcinoma (SCC) and a metastatic small-cell lung carcinoma (SCLC). The sequence analysis of four cDNA libraries revealed 869 individual sequences. Of these, 342 were tested using northern blots of lung cancer cell lines representing the three major subtypes (SCC, adenocarcinoma, SCLC) which confirmed the differential expression of 236 cDNAs. The extended analysis of 31 randomly chosen fragments confirmed the validity of the approach to identify genes associated with lung cancer development. Additionally, five novel full-length cDNA were isolated encoding the microtubule-associated proteins 1A/1B light chain 3, the epithelial V-like antigen 1 (EVA1), the GTP-binding protein SAR1, a new member of the S100-type calcium binding protein family and a new homeobox-containing gene.

Keywords:  Gene expression profile, Suppression subtractive hybridisation, Human non-small cell lung cancer, Small-cell lung cancer, Sequencing, Gene mapping and full-length cDNA

 

PII: S0959-8049(03)00419-2

doi:10.1016/S0959-8049(03)00419-2

European Journal of Cancer
Volume 39, Issue 13 , Pages 1936-1947, September 2003