Gene expression profiles in human non-small and small-cell lung cancers

Received 17 October 2002; received in revised form 6 February 2003; accepted 13 February 2003.

Abstract

Suppression subtractive hybridisation (SSH) was performed comparing normal bronchial epithelial cells with a lung squamous cell carcinoma (SCC) and a metastatic small-cell lung carcinoma (SCLC). The sequence analysis of four cDNA libraries revealed 869 individual sequences. Of these, 342 were tested using northern blots of lung cancer cell lines representing the three major subtypes (SCC, adenocarcinoma, SCLC) which confirmed the differential expression of 236 cDNAs. The extended analysis of 31 randomly chosen fragments confirmed the validity of the approach to identify genes associated with lung cancer development. Additionally, five novel full-length cDNA were isolated encoding the microtubule-associated proteins 1A/1B light chain 3, the epithelial V-like antigen 1 (EVA1), the GTP-binding protein SAR1, a new member of the S100-type calcium binding protein family and a new homeobox-containing gene.

Keywords: Gene expression profile, Suppression subtractive hybridisation, Human non-small cell lung cancer, Small-cell lung cancer, Sequencing, Gene mapping and full-length cDNA

a Institute of Pathology, University Hospital Charité, Schumannsrt. 20/21 Berlin 10098, Germany

b Genetics Department, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA

Corresponding author. Tel.: +49-30-450-536050; fax: +49-30-450-536110

1 Both authors contributed equally to this study.

2 Current address: Experimental Immunology Branch, National Cancer Institute/National Institutes of Health, Bethesda, MD, USA.

PII: S0959-8049(03)00419-2

doi:10.1016/S0959-8049(03)00419-2

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