European Journal of Cancer
Volume 39, Issue 13 , Pages 1881-1887 , September 2003

The study of p16 and p15 gene methylation in head and neck squamous cell carcinoma and their quantitative evaluation in plasma by real-time PCR

  • T.-S. Wong

      Affiliations

    • Department of Surgery, The University of Hong Kong, Hong Kong, PR China
  • ,
  • M.W.-L. Man

      Affiliations

    • Department of Surgery, The University of Hong Kong, Hong Kong, PR China
  • ,
  • A.K.-Y. Lam

      Affiliations

    • Department of Pathology, The University of Hong Kong, Hong Kong, PR China
  • ,
  • W.I. Wei

      Affiliations

    • Department of Surgery, The University of Hong Kong, Hong Kong, PR China
  • ,
  • Y.-L. Kwong

      Affiliations

    • Department of Medicine, The University of Hong Kong, Hong Kong, PR China
  • ,
  • A.P.-W. Yuen

      Affiliations

    • Department of Surgery, The University of Hong Kong, Hong Kong, PR China
    • Corresponding Author InformationCorresponding author. Tel.: +852-2855-4584; fax: +852-2855-3464

Received 4 December 2002 ,Revised 24 February 2003 ,Accepted 6 May 2003.

References 

  1. Michalides RJ, van de Brekel M, Balm F. Defects in G1-S cell cycle control in head and neck cancer (a review). Head Neck. 2002;24:694–704
  2. Namazie A, Alavi S, Olopade OI, et al.  Cyclin D1 amplification and p16(MTS1/CDK4I) deletion correlate with poor prognosis in head and neck tumors. Laryngoscope. 2002;112:472–481
  3. Le Frere-Belda MA, Cappellen D, Daher A, et al.  p15(INK4b) in bladder carcinomas (decreased expression in superficial tumours). Br. J. Cancer. 2001;85:1515–1521
  4. Yuen PW, Man M, Lam KY, Kwong YL. Clinicopathological significance of p16 gene expression in the surgical treatment of head and neck squamous cell carcinomas. J. Clin. Pathol. 2002;55:58–60
  5. Esteller M. CpG island hypermethylation and tumor suppressor genes (a booming present, a brighter future). Oncogene. 2002;21:5427–5440
  6. Esteller M, Herman JG. Cancer as an epigenetic disease (DNA methylation and chromatin alterations in human tumours). J. Pathol. 2002;196:1–7
  7. Kresty LA, Mallery SR, Knobloch TJ, et al.  Alterations of p16(INK4a) and p14(ARF) in patients with severe oral epithelial dysplasia. Cancer Res. 2002;62:5295–5300
  8. Waki T, Tamura G, Tsuchiya T, Sato K, Nishizuka S, Motoyama T. Promoter methylation status of E-cadherin, hMLH1, and p16 genes in nonneoplastic gastric epithelia. Am. J. Pathol. 2002;161:399–403
  9. Issa JP, Ahuja N, Toyota M, Bronner MP, Brentnall TA. Accelerated age-related CpG island methylation in ulcerative colitis. Cancer Res. 2001;61:2577–3573
  10. Kang GH, Shim YH, Jung HY, et al.  CpG island methylation in premalignant stages of gastric carcinoma. Cancer Res. 2001;61:2847–2851
  11. Belinsky SA, Palmisano WA, Gilliland FD, et al.  Aberrant promoter methylation in bronchial epithelium and sputum from current and former smokers. Cancer Res. 2002;62:2370–3277
  12. Wu TL, Zhang D, Chia JH, Tsao KH, Sun CF, Wu JT. Cell-free DNA (measurement in various carcinomas and establishment of normal reference range). Clin. Chim. Acta. 2002;321:77–87
  13. Thijssen MA, Swinkels DW, Ruers TJ, de Kok JB. Difference between free circulating plasma and serum DNA in patients with colorectal liver metastases. Anticancer Res. 2002;22:421–425
  14. Sozzi G, Conte D, Mariani L, et al.  Analysis of circulating tumor DNA in plasma at diagnosis and during follow-up of lung cancer patients. Cancer Res. 2001;61:4675–4678
  15. Jahr S, Hentze H, Englisch S, et al.  DNA fragments in the blood plasma of cancer patients (quantitations and evidence for their origin from apoptotic and necrotic cells). Cancer Res. 2001;61:1659–1665
  16. Esteller M, Sanchez-Cespedes M, Rosell R, et al.  Detection of aberrant promoter hypermethylation of tumor suppressor genes in serum DNA from non-small cell lung cancer patients. Cancer Res. 1999;59:67–70
  17. Hibi K, Taguchi M, Nakayama H, et al.  Molecular detection of p16 promoter methylation in the serum of patients with esophageal squamous cell carcinoma. Clin. Cancer Res. 2001;7:3135–3138
  18. Sanchez-Cespedes M, Esteller M, Wu L, et al.  Gene promoter hypermethylation in tumors and serum of head and neck cancer patients. Cancer Res. 2000;60:892–895
  19. Zou HZ, Yu BM, Wang ZW, et al.  Detection of aberrant p16 methylation in the serum of colorectal cancer patients. Clin. Cancer Res. 2002;8:188–191
  20. Lee TL, Leung WK, Chan MW, et al.  Detection of gene promoter hypermethylation in the tumor and serum of patients with gastric carcinoma. Clin. Cancer Res. 2002;8:1761–1766
  21. Wong IH, Lo YM, Yeo W, Lau WY, Johnson PJ. Frequent p15 promoter methylation in tumor and peripheral blood from hepatocellular carcinoma patients. Clin. Cancer Res. 2000;6:3516–3521
  22. Yuen PW, Lam KY, Chan AC, Wei WI, Lam LK. Clinicopathological analysis of local spread of carcinoma of the tongue. Am. J. Surg. 1998;175:242–244
  23. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB. Methylation-specific PCR (a novel PCR assay for methylation status of CpG islands). Proc. Natl. Acad. Sci. USA. 1996;93:9821–9826
  24. Wong TS, Chang HW, Tang KC, et al.  High frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patients. Clin. Cancer Res. 2002;8:433–437
  25. Eads CA, Lord RV, Wickramasinghe K, et al.  Epigenetic patterns in the progression of esophageal adenocarcinoma. Cancer Res. 2001;61:3410–3418
  26. Waber P, Dlugosz S, Cheng QC, Truelson J, Nisen PD. Genetic alterations of chromosome band 9p21-22 in head and neck cancer are not restricted to p16INK4a. Oncogene. 1997;15:1699–1704
  27. Lang JC, Tobin EJ, Knobloch TJ, et al.  Frequent mutation of p16 in squamous cell carcinoma of the head and neck. Laryngoscope. 1998;108:923–928
  28. Leung WK, Yu J, Ng EK, et al.  Concurrent hypermethylation of multiple tumor-related genes in gastric carcinoma and adjacent normal tissues. Cancer. 2001;15:2294–2301
  29. Califano J, van der Riet P, Westra W, et al.  Genetic progression model for head and neck cancer (implications for field cancerization). Cancer Res. 1996;56:2488–2492
  30. Leon X, Quer M, Diez S, et al.  Second neoplasm in patients with head and neck cancer. Head Neck. 1999;21:204–210
  31. Kim DH, Nelson HH, Wiencke JK, et al.  p16(INK4a) and histology-specific methylation of CpG islands by exposure to tobacco smoke in non-small cell lung cancer. Cancer Res. 2001;61:3419–3424
  32. Papadimitrakopoulou VA, Izzo J, Mao L, et al.  Cyclin D1 and p16 alterations in advanced premalignant lesions of the upper aerodigestive tract (role in response to chemoprevention and cancer development). Clin. Cancer Res. 2001;7:3127–3134

PII: S0959-8049(03)00428-3

doi: 10.1016/S0959-8049(03)00428-3

European Journal of Cancer
Volume 39, Issue 13 , Pages 1881-1887 , September 2003